The auction house launched specialist sales in this category in January after noting some encouraging results for urban art as part of its 20th century, contemporary art and design auctions.
The January sale was led by head of department James Nurse and consultant Nick Clark and generated a hammer total of £250,000.
Chiswick recorded its first ever £1m sale earlier this month with its two day Asian art sale.
The June 20 sale will offer 318 lots and the majority of the top ten lots on offer at the urban art sale are works by Banksy (b.1974). These include the highest estimated lot at the auction – Stop And Search, a 2ft 6in x 22.5in (77 x 58cm) screenprint from 2007.
From an edition of 500, it is signed in blue pencil and comes with a Pest Control certificate of authenticity. The estimate is £35,000-40,000.
Also at the sale is a 2009 screenprint of No Ball Games (Green) which comes from an edition of 250. Also signed and with Pest Control authentication, the 2ft 10in x 2ft 11in (86 x 88cm) print is estimated at £30,000-35,000.