Estimated at $400,000, the price is an auction record for a Marvel Comics Silver or Bronze Age cover. The hammer price was $400,000.
The cover is by artists John Romita Sr and Frank Giacoia in ink over blue pencil on World Color Bristol board and the price achieved is also the most expensive for an artwork by Romita Sr.
The previous record for was set by Heritage in 2013 when a 1973 cover sold for $286,800 (£204,400).
Heritage Auctions’ senior vice president Ed Jaster said: “This cover was done during the peak period of John Romita, Sr’s artwork, at a time when Spider-Man’s popularity was extremely high.”
Other top-sellers included a copy of Batman #1 DC Comics (1940), estimated at $250,000, which sold for a premium inclusive $334,600 (£238,400) ($280,000 hammer price), and the first appearance of Green Lantern from All-American Comics #16 for DC Comics (1940), which sold for a premium-inclusive $215,100 (£153,314) (hammer price $180,000), above its $125,000 estimate.